Lysine production from biomass-based sugar platform
Main ideas and research focus for Lysine production
Lysine is used extensively world-wide in feed production, and is currently produced commercially via fermentation of sugars from first generation feedstocks. The typical production organism is however unable to convert C5 sugars and respires sugar to CO2along the process. Therefore, new production organisms for more efficient bioconversion of both C5 and C6 sugar streams from biomass into lysine are required to make the production more cost-effective. A cost competitive process would open for a larger market, partly by replacing high protein feeds like soybeans
Two routes are suggested for developing the microbial processes: (i) C. glutamicum is engineered for xylose utilization, increased inhibitor and product tolerance and to ensure an optimal supply of NADPH. (ii) A new process is developed based e.g. on lactic acid bacteria (LAB).
Figure 1: An overview of the strategies for both routes for developing the microbial processes.
- To develop and demonstrate a commercially relevant process for production of L-lysine, based on second generation feedstocks, which is able to compete, or in the future will be able to compete, with existing processes based on first generation sugars
Publications in project 4:
Wang, Z., Liu, J., Chen, L., Zheng, A-P, Solem, C., Jensen, PR. 2018. Alterations in the transcription factors GntR1 and RamA enhance the growth and central metabolism of Corynebacterium glutamicum. Metabolic Engineering, Volume 48, 1-12.
Wang, Z., Hung Joshua Chan, S., Sudarsan, S., Blank, L.M., Jensen, P.R., Solem, C.: Elucidation of the regulatory role of the fructose operon reveals a novel target for enhancing the NADPH supply in Corynebacterium glutamicum, Metabolic Engineering, Volume 38, November 2016, Pages 344-357.
Wang, Z.; Moslehi-Jenabian, S.; Solem, C.; Jensen, P.R.: Increased expression of pyruvate carboxylase and biotin protein ligase increases lysine production in a biotin prototrophicCorynebacterium glutamicum strain, Engineering in Life Sciences, January 2015, Volume 15, Issue 1.