PRELIMINARY RESULTS AND STATUS OF PROJECT 4, MARCH 2017
In project 4, we have successfully constructed a C. glutamicum mutant which could metabolize xylose. For further improving the xylose consumption, we introduced an additional transport system which improved the xylose metabolism significantly. The strain metabolize glucose and xylose simultaneously and the production of lysine was maintained. We conducted adaptive evolution experiments to improve growth further and we now have mutants with efficient xylose consumption and fast growth.
We have previously demonstrated the improvement of toxicity tolerance for C. glutamicum in lignocellulose hydrolysate medium and we will now work on combining these properties in a single strain C. glutamicum, capable of producing lysine from lignocellulose hydrolysate.