Project 2:
Products from green biomass (protein, storable fibres, inorganic elements)

Main ideas and research focus for green biomass work in Biovalue

The overall vision is to develop a decentralised, robust and overall optimised pretreatment process for green biomass for production of animal protein feed and a storable fibre fraction that can be used as substrate on centralised biorefineries.

In order to achieve viable processes on leaf protein extraction from biomass, it is crucial to integrate and optimise the whole process, e.g. wet fractionation, filtration recovery input and energy costs against product yield and quality. The partnering companies KMC and Hamlet Protein have both established full-scale industrial plants for protein extraction from potatoes and soybean, respectively. KMC has made its potato protein process line available to the project for large scale green biomass test runs.


  1. To characterise how the composition of different types of green biomass and processing conditions such as temperature, pressure, water content and processing time affect the yield and quality of extracted protein and storable fibres
  2. To understand how unwanted secondary plant metabolites compromising feed quality or having an adverse effect on animal production, health or product quality can been removed or deactivated


Damborg, VK, Stødkilde, L, Jensen, SK, Weisbjerg, MR. 2018. Protein value and degradation characteristics of pulp fibre fractions from screw pressed grass, clover, and lucerne. Animal Feed Science and Technology 244, 93-103.

Chowdhury, MR, Lashkari, S, Jensen, SK, Ambye-Jensen, M, Weisbjerg, MR. 2018. Effects of Heat Treatment of Green Protein on in Situ Protein Disappearance and in Vitro Fatty Acid Biohydrogenation. Journal of Agricultural and Food Chemistry 66, 8169-8178.

Corona, A, Ambye-Jensen, M, Vega, GC, Hauschild, MZ, Birkved, M. 2018. Techno-environmental assessment of the green biorefinery concept : Combining process simulation and life cycle assessment at an early design stage. Science of the Total Environment 635, 100-111.

Corona, A, Parajuli, R, Ambye-Jensen, M, Hauschild, MZ, Birkved, M. 2018. Environmental screening of potential biomass for green biorefinery conversion. Journal of Cleaner Production 189, 344-357.

SEGES-produced report by Erik Fog, 2017/18: “Farmer-survey om at indgå i grøn bioraffinering

Dahl-Lassen, R., van Hecke, J., Jørgensen, H., Bukh, C., Andersen, B., Schjoerring, J.K., 2018. High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. Plant Methods 14, 8.

Dotsenko, G., Meyer, A. S., Canibe, N., Thygesen, A., Nielsen, M. K., & Lange, L. (2017). Enzymatic production of wheat and ryegrass derived xylooligosaccharides and evaluation of their in vitro effect on pig gut microbiota. Biomass Conversion and Biorefinery, 11. DOI: 10.1007/s13399-017-0298-y

Stødkilde, L., Damborg, V. K., Jørgensen, H., Lærke, H. N. and Jensen, S. K. (2017), White clover fractions as protein source for monogastrics: dry matter digestibility and protein digestibility-corrected amino acid scores. J. Sci. Food Agric. doi:10.1002/jsfa.8744

Dotsenko, G.; Lange, L.: Enzyme Enhanced Protein Recovery from Green Biomass Pulp. Waste Biomass Valor, Oct 2016. doi:10.1007/s12649-016-9718-7

Hermansen, J.E; Jørgensen, U; Lærke, P.E; Manevski, K; Boelt, B; Jensen, S.K; Weisbjerg, M.R; Dalsgaard, T.K; Danielsen, M; Asp, T; Ambye-Jensen, M; Sørensen, C.A.G; Jensen, M.V; Gylling, M; Lindedam, J; Lübeck, M; Fog, E, 2017. Green biomass – protein producion through bio_refining. DCA – Nationalt Center for Fødevarer og Jordbrug. 72 p.

Dotsenko, G.; Nielsen, M. K.; Lange, L.: Statistical model semiquantitatively approximates arabinoxylooligosaccharides’ structural diversity. Carbohydrate Research, Volume 426, Maj 2016, Pages 9-14

Dotsenko, G.; Tong, X.; Pilgaard, B.; Busk, P. K.; Lange, L.: Acidic–alkaline ferulic acid esterase from Chaetomium thermophilum var. dissitum: Molecular cloning and characterization of recombinant enzyme expressed in Pichia pastoris. Biocatalysis and Agricultural Biotechnology, Volume 5, January 2016, Pages 48–55

Grøn biomasse i Danmark (Inkl. casebeskrivelse af BioValue), Det Nationale Bioøkonomi Panel, 2015

Grøn Biomasse, DCA rapport nr. 068, September 2015: Termansen et al.